Journal: Sensors (Basel, Switzerland)

Article Title: A Red-Emitting Fluorescence Sensor for Detecting Boronic Acid-Containing Agents in Cells

doi: 10.3390/s22197671

Figure Lengend Snippet: Representative fluorescence images of T3M-4 cells: ( A – C ) BPA-present group, ( D – F ) BPA-absent group. ( A , D ) Fluorescence images after 30 min incubation with BS-631 (100 μM) (GFP filter: Ex: 470/40 nm, Em: 525/50 nm). ( B , E ) Nucleus staining using Hoechst 33342 (DAPI filter: Ex: 360/40 nm, Em: 460/50 nm). ( C , F ) Merged images of fluorescence from BS-631 and Hoechst 33342.

Article Snippet: Fluorescence images were filtered using a GFP filter cube (Keyence OP-87763, Ex: 470/40 nm, Em: 525/50 nm, pseudo-color: green) after the addition of BS-631 to cells with or without BPA, as shown in .

Techniques: Fluorescence, Incubation, Staining

Journal: Small Science

Article Title: A Microfluidic Platform for Actin‐Based Membrane Remodeling Reveals the Stabilizing Role of Branched Actin Networks on Lipid Microdomains

doi: 10.1002/smsc.202500210

Figure Lengend Snippet: Design of the microfluidic device and experimental strategy. A) Scheme of the microfluidic chamber, where the larger circle on the right is the inlet channel, and the smaller one on the left the outlet; B) C‐like traps composed of four micropillars; C) three‐step experimental strategy: GUV filling, incubation of NPFs on the GUVs, and branched Arp2/3‐nucleated F‐actin network polymerization. D) GUVs loading and entrapment: bright‐field and epifluorescence images of DOPC GUVs in microtraps, scale bar: 100 μm; and E) close‐up of an individual trapped GUV, scale bar: 20 μm.

Article Snippet: Lipids and proteins are imaged using a Zeiss Axio observer 7 inverted epifluorescence microscope (GFP filter cube, excitation 470 nm, emission 525 nm; Texas‐red filter cube: excitation 545−580 nm, emission 615 nm) equipped with either a 10X air or a 63X‐1.4 numerical aperture oil‐immersion objective.

Techniques: Incubation